Coding

Part:BBa_K3747000

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-20)


pMMO-B1. Particulate Methane Monooxygeanse substructure B1

pMMO active site mimic substructure pMMO-B1. Taken from Kim et al 2019 "Biological conversion of methane to methanol through genetic reassembly of native catalytic domains"

Basic part, consult BBa_K3747003 for composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 19
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 325


references

Kim, Hyun Jin; Huh, June; Kwon, Young Wan; Park, Donghyun; Yu, Yeonhwa; Jang, Young Eun; Lee, Bo-Ram; Jo, Eunji; Lee, Eun Jung; Heo, Yunseok; Lee, Weontae; Lee, Jeewon (2019). Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nature Catalysis, (), –. doi:10.1038/s41929-019-0255-1

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